Rna Library Prep Protocol
A Rapid Simple And Inexpensive Method For The Preparation Of Strand Specific Rna Seq Libraries High Throughput Sequencing O Gene Expression Library Method
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Library preparation for the illumina sequencing platform requires inputs of a defined length therefore fragmentation of dna or the use of cdna prepared from rna is the starting point.
Rna library prep protocol. New england biolabs offers a series of highly pure reagents that facilitate library preparation of total rna single cell low input rna and small rna for next generation sequencing. The small rna seq library prep kit provides a protocol for generating small rna libraries for illumina sequencing directly from total rna or enriched small rna. A high performing fast and integrated workflow for sensitive applications such as human whole genome sequencing. Library prep kit selector.
Illumina library prep protocols accommodate a range of throughput needs from lower throughput protocols for small labs to fully automated library preparation workstations for large laboratories or genome centers. Please see the primers process at the bottom of the protocol for a better description of primers. The nebnext ultra rna library prep kit for illumina contains reagents for preparation of non directional rna libraries for illumina sequencing. Iv document 15004197v02 document date descriptionofchange part 15004197rev e february 2013 movedworkflowdiagramtostartofprotocol.
Pre mixed reagents are ready to use at every step eliminating the need for manual master mix preparation. Rapid library preparation from a broad range of sample types for studying the coding and non coding transcriptome with unparalleled study flexibility. It s a good idea to test some rna from the same source as the. Rna and small rna libraries are prepared using different workflows that are tailored to the downstream sequencing platform that they will be used with.
Multiplexing of up to 96 samples multiplexing of up to 96 samples is possible with complimentary i7 indexes provided in the kit. This total rna seq library prep kit s protocol is fragmentation free and works with up to 2 ug of undepleted total rna down to 50 ng of undepleted total rna or as little as 5 ng of ribosome depleted rna samples.
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